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제목
미세 패턴이 새겨진 유리 기판은 마이크로컨택트 프린팅을 통해 제작되었습니다. 패턴 스탬프를 만들기 위해, 점성이 있는 폴리디메틸실록산(PDMS)(Sylgard 184; Avantor, Radnor, PA, 단량체/가교제 비율 10:1)을 패턴이 새겨진 실리콘 웨이퍼 위에 붓고 30분 동안 탈기한 후 80°C에서 2시간 동안 경화시켰습니다. 경화된 PDMS 엘라스토머 조각을 잘라내어 패턴면이 위를 향하도록 플라스틱 접시에 보관한 후 스탬프로 사용했습니다. 스탬프는 70% 에탄올로 한 번 세척하고 질소 가스를 약하게 흘려주면서 건조시켰습니다. 스탬프를 플라즈마 세척기(Zepto-QRS 200; Diener Electronic, Ebhausen, Germany)를 사용하여 100% 출력으로 30초간 세척한 후, 피브리노겐 Alexa Fluor 594(#F13193; Thermo Fisher Scientific, Waltham, MA) 잉크 용액 100 μL(Dulbecco's phosphate-buffered saline [PBS] [#D8537; Sigma-Aldrich, St. Louis, MO]에 57.7 μg/mL 농도로 용해)를 도포했습니다. 그런 다음 스탬프를 파라필름으로 빠르게 덮고 37°C에서 30분간 배양했습니다. 파라필름을 제거하고 스탬프를 탈이온수로 한 번 세척한 후, 질소 가스를 약하게 흘려주면서 건조했습니다. 건조되고 잉크가 묻은 스탬프를 플라즈마 세척(30초, 100% 출력)된 유리 바닥 접시(35mm, #81218; ibidi, Graefelfing, 독일)에 무늬가 있는 면이 아래쪽을 향하도록 놓았습니다. 37°C에서 15분간 배양한 후, 스탬프를 접시에서 조심스럽게 떼어내자 사용한 스탬프의 무늬를 그대로 반영하는 미세 무늬가 남았습니다. 미세 무늬가 새겨진 접시는 탈이온수로 세 번 세척했습니다. 보관을 위해 접시에 탈이온수 1mL를 추가했습니다. 미세 무늬가 새겨진 접시는 며칠 동안 사용할 수 있었습니다. <a 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